2H85
Crystal Structure of Nsp 15 from SARS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-11-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97938 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 166.584, 166.584, 166.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.700 - 2.600 |
R-factor | 0.2092 |
Rwork | 0.208 |
R-free | 0.24008 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.301 |
Data reduction software | MOSFLM |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | 2.667 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.169 | 0.778 |
Number of reflections | 24505 | |
<I/σ(I)> | 11.8 | 4 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 11.8 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 297 | 5-20% PEG 10000, 0.2M imidazole malate pH 9.0-10.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |