2H6D
Protein Kinase Domain of the Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK alpha-2 chain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-05-19 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.454, 67.410, 50.493 |
Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
Resolution | 19.720 - 1.850 |
R-factor | 0.19003 |
Rwork | 0.188 |
R-free | 0.22674 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fh9 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.513 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.720 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 22387 | |
<I/σ(I)> | 26.91 | 1.6 |
Completeness [%] | 98.8 | 83 |
Redundancy | 3.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 18.6% PEG 4000, 0.1M AmSO4, 0.1M Tris-HCl pH 8.5, 15%v/v isopropanol,5mM ATP/MgCl, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |