2H6D
Protein Kinase Domain of the Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK alpha-2 chain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.454, 67.410, 50.493 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 19.720 - 1.850 |
| R-factor | 0.19003 |
| Rwork | 0.188 |
| R-free | 0.22674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fh9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 22387 | |
| <I/σ(I)> | 26.91 | 1.6 |
| Completeness [%] | 98.8 | 83 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 18.6% PEG 4000, 0.1M AmSO4, 0.1M Tris-HCl pH 8.5, 15%v/v isopropanol,5mM ATP/MgCl, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
