2H66
The Crystal Structure of Plasmodium Vivax 2-Cys peroxiredoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.550, 149.590, 131.910 |
Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
Resolution | 41.560 - 2.500 |
R-factor | 0.1963 |
Rwork | 0.194 |
R-free | 0.23205 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qmv |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.560 | 2.530 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.115 | 0.488 |
Number of reflections | 86441 | |
<I/σ(I)> | 10.8 | 1.3 |
Completeness [%] | 99.2 | 99.8 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 5% PEG 4000, 50 mM NaAc, 100 mM NaAC, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |