2H5Z
Crystallographic structure of digestive lysozyme 1 from Musca domestica bound to chitotetraose at 1.92 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.953, 78.809, 44.792 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.920 |
| R-factor | 0.17843 |
| Rwork | 0.175 |
| R-free | 0.24247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fbd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.660 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.990 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 16455 | |
| <I/σ(I)> | 16.2 | 2.4 |
| Completeness [%] | 88.1 | 82 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1% PEG 400, 0.1M sodium Hepes, 1.4M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
