2H5U

Crystal structure of laccase from Cerrena maxima at 1.9A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	200
Detector technology	CCD
Collection date	2005-04-12
Detector	MAR CCD 165 mm
Wavelength(s)	0.8068
Spacegroup name	P 21 21 21
Unit cell lengths	52.581, 77.101, 130.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.270 - 1.900
R-factor	0.19193
Rwork	0.190
R-free	0.23835
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.069
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.270	1.950
High resolution limit [Å]	1.900	1.900
Number of reflections	39919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	50 mM sodium citrate, 15% PEG 6000, 0.04M tris-malein-NaOH buffer, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K