2H5Q
Crystal structure of mCherry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.762, 42.855, 61.057 |
| Unit cell angles | 90.00, 112.31, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.360 |
| R-factor | 0.1486 |
| Rwork | 0.147 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 47050 | |
| Completeness [%] | 100.0 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM NaOAc, 100 mM Tris pH 8.5, 30% PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
