2H52
Crystal structure of human bisphosphoglycerate mutase complex with 3-phosphoglycerate (18 days)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-08-29 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.810, 71.250, 160.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.490 - 2.000 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h4z |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 | 0.269 |
Number of reflections | 37816 | |
<I/σ(I)> | 24.1 | 7.6 |
Completeness [%] | 97.7 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 289 | PEG6000, HEPES, VAPOR DIFFUSION, HANGING DROP, pH 7.50, temperature 289K |