2H4T
Crystal structure of rat carnitine palmitoyltransferase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-14 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.770, 73.800, 90.560 |
| Unit cell angles | 70.65, 73.62, 88.06 |
Refinement procedure
| Resolution | 28.700 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (THEN SOLVE/RESOLVE) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.402 |
| Number of reflections | 126888 | |
| <I/σ(I)> | 10.7986 | 1.741 |
| Completeness [%] | 90.7 | 72.3 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 100 mM Hepes, 15% Tacsimate, 16% PEG 5000MME, 1% Benzyldimethyldodecyl ammonium bromide (BAM), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
