2H4M
Karyopherin Beta2/Transportin-M9NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.013, 154.093, 141.667 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.050 |
| Rwork | 0.238 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qbk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 59061 | |
| Completeness [%] | 95.1 | 0.7479 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 40 mM MES pH 6.5, 3M potassium formate and 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
