2H4L

Complex of PMM/PGM with ribose 1-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Wavelength(s)	1.0398
Spacegroup name	P 21 21 21
Unit cell lengths	70.717, 74.095, 84.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.850 - 2.400
R-factor	0.18998
Rwork	0.186
R-free	0.25946
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1p5d
RMSD bond length	0.009
RMSD bond angle	1.192
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	16595
Completeness [%]	91.7	83.9
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	NA, K TARTRATE , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K