2H3R

Crystal structure of ORF52 from Murid herpesvirus 4 (MuHV-4) (Murine gammaherpesvirus 68). Northeast Structural Genomics Consortium target MhR28B.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912,0.97947,0.96790,0.97930
Spacegroup name	P 1 2 1
Unit cell lengths	54.926, 49.240, 88.915
Unit cell angles	90.00, 105.61, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.275
Rwork	0.275
R-free	0.29500
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.086	0.394
Number of reflections	23521
<I/σ(I)>	13.8
Completeness [%]	93.9	93.2
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	80% PEG 400, 100mM MOPS, 100mM NaNO3, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00
1	VAPOR DIFFUSION, HANGING DROP	7	293	80% PEG 400, 100mM MOPS, 100mM NaNO3, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00
1	VAPOR DIFFUSION, HANGING DROP	7	293	80% PEG 400, 100mM MOPS, 100mM NaNO3, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00