2H3B
Crystal Structure of Mouse Nicotinamide Phosphoribosyltransferase/Visfatin/Pre-B Cell Colony Enhancing Factor 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-12-03 |
| Wavelength(s) | 0.9797,0.9796,1.0107 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.750, 107.490, 82.960 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 36.230 - 1.950 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 77921 | |
| <I/σ(I)> | 17.9 | 3.6 |
| Completeness [%] | 99.6 | 96.3 |
| Redundancy | 7.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 291 | 5 mg/ml Se-Met LM1467 with 10 mM added nicotinamide, reservoir solution containing 0.1 M Tris pH 8.2, 0.2 M MgSO4, and 14% PEG8000, and acetone in a 1:0.6:0.4 ratio in a total initial drop volume of 2 microliter., VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.20 |
