2H36
Structure of ORF14 from Sulfolobus Islandicus Filamentous Virus (SIFV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 68.099, 68.099, 132.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.070 - 2.950 |
| R-factor | 0.23353 |
| Rwork | 0.227 |
| R-free | 0.29442 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.428 |
| Data reduction software | MOSFLM |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.070 | 0.386 |
| Number of reflections | 4170 | |
| <I/σ(I)> | 75 | 17 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 53.4 | 55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 18-26% (w/v) polyethylene glycol 4000, 0.5M MgCl2, 33mM HEPES, 0.2M imidazole malate pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.3 |
