2H30
Crystal structure of the N-terminal domain of PilB from Neisseria gonorrhoeae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2003-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790,0.9791,0.9500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.381, 51.421, 80.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 1.600 |
Rwork | 0.188 |
R-free | 0.20900 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.850 |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.042 | 0.184 |
Number of reflections | 24399 | |
<I/σ(I)> | 25 | 7.9 |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | Equal volumes of protein (30 mg mL-1 in 20 mM Hepes pH 7.5, 100 mM NaCl) and well solutions (0.1 M Mes pH 6.5, 0.2 M NH4SO4, 26% PEG 2000 MME), VAPOR DIFFUSION, SITTING DROP, temperature 277K |