2H2W

Crystal structure of Homoserine O-succinyltransferase (EC 2.3.1.46) (Homoserine O-transsuccinylase) (HTS) (tm0881) from THERMOTOGA MARITIMA at 2.52 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	72.609, 72.609, 126.913
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.300 - 2.520
Rwork	0.190
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ghr
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.300	50.000	2.610
High resolution limit [Å]	2.520	5.430	2.520
Rmerge	0.046	0.034	0.387
Number of reflections	13643	1483	1327
<I/σ(I)>	18.5		6.1
Completeness [%]	99.9		99.7
Redundancy	10.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8	293	10.0% iso-Propanol, 0.1M Imidazole pH 8.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K