2H2T
CD23 Lectin domain, Calcium 2+-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2004-03-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.02336 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.310, 51.261, 58.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.630 - 1.300 |
R-factor | 0.16953 |
Rwork | 0.168 |
R-free | 0.20045 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h2r |
RMSD bond length | 0.017 |
RMSD bond angle | 1.658 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 30523 | |
<I/σ(I)> | 41.6 | 3.5 |
Completeness [%] | 99.4 | 100 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 17% (w/v) PEG 4000, 100 mM Mes buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |