2H2R
Crystal structure of the human CD23 Lectin domain, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2003-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97895 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.354, 50.667, 75.061 |
| Unit cell angles | 90.00, 127.09, 90.00 |
Refinement procedure
| Resolution | 59.870 - 1.500 |
| R-factor | 0.12562 |
| Rwork | 0.124 |
| R-free | 0.16481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A MODIFIED VERSION OF PDB ENTRY 1DV8 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.449 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 53947 | |
| <I/σ(I)> | 18.7 | 6.3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 1.2 M sodium monobasic phosphate, 0.8 M potassium dibasic phosphate, 0.1 M Caps buffer pH 10.5, and 0.2 M lithium sulfate; final pH 6.2 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
