2H2K
Crystal Structure Analysis of Human S100A13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.196, 60.665, 78.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k96 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.370 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.229 |
| Number of reflections | 13373 | |
| Completeness [%] | 98.2 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 25% PEGmme 550, 10mM CaCl2, 0.1M Na-Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
