2H2E
Structure of Rubisco LSMT bound to AzaAdoMet and Lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-08-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9686 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 132.630, 159.490, 267.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.730 - 2.600 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 85610 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.2 | 98.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 100 mM NaCitrate pH 6.8, 1.43-1.74 M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
