2H2E
Structure of Rubisco LSMT bound to AzaAdoMet and Lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-08-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9686 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 132.630, 159.490, 267.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.730 - 2.600 |
R-factor | 0.262 |
Rwork | 0.262 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.800 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 85610 | |
<I/σ(I)> | 7.2 | |
Completeness [%] | 98.2 | 98.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 100 mM NaCitrate pH 6.8, 1.43-1.74 M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |