2H28
Crystal structure of YeeU from E. coli. Northeast Structural Genomics target ER304
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.216, 45.310, 60.185 |
| Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| Rwork | 0.220 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.325 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.515 |
| Number of reflections | 35928 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 96.9 | 87.3 |
| Redundancy | 2.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under oil | 7 | 293 | MOPS, 0.1M MgSO4, 20% PEG 4000, pH 7.0, Under oil, temperature 293K |
