2H23
Structure of Rubisco LSMT bound to Trimethyllysine and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9686 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 131.860, 157.550, 267.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.450 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 101323 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 100 mM NaCitrate pH 6.8, 1.17-1.41 M NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
