2H1P
THE THREE-DIMENSIONAL STRUCTURES OF A POLYSACCHARIDE BINDING ANTIBODY TO CRYPTOCOCCUS NEOFORMANS AND ITS COMPLEX WITH A PEPTIDE FROM A PHAGE DISPLAY LIBRARY: IMPLICATIONS FOR THE IDENTIFICATION OF PEPTIDE MIMOTOPES
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1995-09 |
Detector | SIEMENS |
Spacegroup name | P 1 |
Unit cell lengths | 43.790, 51.250, 60.180 |
Unit cell angles | 91.82, 98.57, 107.84 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.186 * |
Rwork | 0.186 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1FLR (FAB 4-4-20) |
RMSD bond length | 0.017 |
RMSD bond angle | 1.850 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | X-PLOR |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.065 | |
Number of reflections | 19345 | |
<I/σ(I)> | 15.3 | |
Completeness [%] | 90.0 | 79 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | 18% PEG 8K, PH 8.5, 1% GLYCEROL |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 18 (%) | |
2 | 1 | reservoir | Tris | 0.1 (M) | |
3 | 1 | reservoir | glycerol | 1 (%) |