2H1O
Structure of FitAB bound to IR36 DNA fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.040, 82.403, 135.503 |
Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
Resolution | 70.360 - 3.000 |
R-factor | 0.269 |
Rwork | 0.212 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h1c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.360 | 3.140 |
High resolution limit [Å] | 2.990 | 2.990 |
Number of reflections | 33243 | |
<I/σ(I)> | 3.1 | 44 |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M sodium acetate, pH 4.0, 0.27 M sodium acetate, pH 7.0, 7.2 % PEG 20,000, 7.2 % PEG monomethyl ether 550, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium acetate | ||
2 | 1 | 1 | PEG | ||
3 | 1 | 1 | PEG monomethyl ether | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | sodium acetate | ||
6 | 1 | 2 | PEG | ||
7 | 1 | 2 | PEG monomethyl ether |