2H1M
Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.978948 |
Spacegroup name | H 3 |
Unit cell lengths | 46.314, 46.314, 128.348 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.21352 |
Rwork | 0.212 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Similar RNA structure from the crystal grown in different conditions. |
RMSD bond length | 0.005 |
RMSD bond angle | 1.125 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 2227 | |
<I/σ(I)> | 12.7 | 6.5 |
Completeness [%] | 97.2 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 1.0 M Li2SO4, 0.1 M Na-citrate pH 5.6, 0.5 M ((NH4))2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Li2SO4 | ||
2 | 1 | 1 | Na-citrate | ||
3 | 1 | 1 | (NH4))2SO4 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Li2SO4 | ||
6 | 1 | 2 | Na-citrate | ||
7 | 1 | 2 | H2O |