2H1E
Tandem chromodomains of budding yeast CHD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-12 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 136.185, 136.185, 57.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.570 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2y CHAIN A WITHOUT B1-B2 LOOP |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.570 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25838 | |
| <I/σ(I)> | 10.8 | 1.7 |
| Completeness [%] | 95.7 | 80.9 |
| Redundancy | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 283 | 10mM BTP, 12mM NaCl, 5mM TCEP, 0.9M ammonium sulfate, 3.75% isopropanol, 4.5mg/ml yeast CHD1 tandem chromodomains, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00 |
