2H1C
Crystal Structure of FitAcB from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.970, 50.700, 48.280 |
| Unit cell angles | 90.00, 118.55, 90.00 |
Refinement procedure
| Resolution | 39.110 - 1.800 |
| R-factor | 0.223 |
| Rwork | 0.191 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.110 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 13340 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 96.5 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | 0.26 M sodium phosphate/citrate pH 4.7 and 2.0 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | 0.26 M sodium phosphate/citrate pH 4.7 and 2.0 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | 0.26 M sodium phosphate/citrate pH 4.7 and 2.0 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
