2H0J
Crystal structure of PucM in the presence of 5,6-diaminouracil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-16 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 0.97144 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 72.956, 72.956, 145.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.27100 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.107 | 0.041 | 0.546 |
Number of reflections | 9261 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 6.7 | 5.7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 295 | 0.1M ammonium acetate, 0.5M NaCl, 0.5M ammonium sulfate, pH 4.2, VAPOR DIFFUSION, temperature 295K |