2H0D

Structure of a Bmi-1-Ring1B Polycomb group ubiquitin ligase complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2821, 1.2826, 1.25
Spacegroup name	P 63
Unit cell lengths	120.514, 120.514, 27.209
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	104.260 - 2.500
R-factor	0.21137
Rwork	0.210
R-free	0.24418
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.319
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.066	0.258
Number of reflections	8143
<I/σ(I)>	15.9
Completeness [%]	99.1	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	20 mM Tris, 500 mM NaCl, 1 mM EDTA, 1.5 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	8	290	20 mM Tris, 500 mM NaCl, 1 mM EDTA, 1.5 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	8	290	20 mM Tris, 500 mM NaCl, 1 mM EDTA, 1.5 mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K