2H0A

Crystal structure of probable Transcription regulator from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00, 0.9789, 0.9793, 0.9950
Spacegroup name	P 41 21 2
Unit cell lengths	74.450, 74.450, 138.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 2.800
R-factor	0.224
Rwork	0.224
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.500
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.086	0.539
Number of reflections	22920
Completeness [%]	100.0	100
Redundancy	10.2	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Ethanol, Tris, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K