2H09

Crystal structure of diphtheria toxin repressor like protein from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	298
Detector technology	CCD
Collection date	2006-03-30
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	75.760, 75.760, 41.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.100
R-factor	0.238
Rwork	0.238
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.006	0.329
Number of reflections	8314
Completeness [%]	99.4	95.7
Redundancy	9.5	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	PEG 300, Phosphate citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	PEG 300, Phosphate citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K