2H06
Crystal structure of human phosphoribosyl pyrophosphate synthetase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | H 3 |
| Unit cell lengths | 169.683, 169.683, 61.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.980 - 2.200 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dkr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | |
| Number of reflections | 33517 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.15 | 293 | 0.1M citric acid, 1.3M ammonium sulfate, 0.02M sodium dihydrogen phosphate, 0.4M sodium chloride, pH 4.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
