2GZW
Crystal structure of the E.coli CRP-cAMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-03-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 45.370, 57.150, 90.740 |
Unit cell angles | 91.25, 101.95, 103.70 |
Refinement procedure
Resolution | 19.930 - 2.210 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i5z |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.097 | 0.246 |
Number of reflections | 39942 | |
Completeness [%] | 92.1 | 96.6 |
Redundancy | 1.6 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | MG ACETATE, AMMONIUM ACETATE, PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |