2GZM

Crystal Structure of the Glutamate Racemase from Bacillus anthracis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.91840
Spacegroup name	P 1 21 1
Unit cell lengths	56.346, 90.518, 116.799
Unit cell angles	90.00, 100.02, 90.00

Refinement procedure

Resolution	20.000 - 1.990
R-factor	0.203
Rwork	0.203
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1B74 fragments
RMSD bond length	0.009
RMSD bond angle	1.422
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	1.990	1.990
Rmerge	0.125	0.557
Number of reflections	77916
<I/σ(I)>	7.47	2.1
Completeness [%]	94.5	91.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	Protein conc of 8 mG/mL, 50mM sodium glutamate, 2mM DTT, 5% PEG6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K