2GYQ
YcfI, a putative structural protein from Rhodopseudomonas palustris.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-27 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97883 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.451, 101.125, 103.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.700 - 1.400 |
| R-factor | 0.14 |
| Rwork | 0.140 |
| R-free | 0.17570 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.528 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.700 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.059 | 0.513 |
| Number of reflections | 64970 | |
| <I/σ(I)> | 22.6 | 1.82 |
| Completeness [%] | 92.1 | 54.3 |
| Redundancy | 5.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 50 mM zinc acetate, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
