2GXU
HERA N-terminal domain in complex with orthophosphate, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 140 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5419 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.554, 53.655, 44.671 |
| Unit cell angles | 90.00, 95.29, 90.00 |
Refinement procedure
| Resolution | 30.820 - 1.670 |
| R-factor | 0.16914 |
| Rwork | 0.167 |
| R-free | 0.20971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.546 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 21117 | |
| <I/σ(I)> | 25.1 | 2.3 |
| Completeness [%] | 96.1 | 64.4 |
| Redundancy | 7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 14% PEG6000, 0.3M CaCl2, 0.1M Tris/HCl pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
