2GXS

HERA N-terminal domain in complex with AMP, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	140
Detector technology	IMAGE PLATE
Collection date	2006-03-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5419
Spacegroup name	C 1 2 1
Unit cell lengths	136.564, 42.838, 68.645
Unit cell angles	90.00, 100.06, 90.00

Refinement procedure

Resolution	50.000 - 1.660
R-factor	0.19172
Rwork	0.189
R-free	0.23942
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.710
Data scaling software	HKL-2000
Phasing software	COMO
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.720
High resolution limit [Å]	1.660	1.660
Number of reflections	45511
<I/σ(I)>	27.5	1.9
Completeness [%]	97.6	85.6
Redundancy	4.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	295	14% PEG6000, 0.3M CaCl2, 0.1M Tris/HCl pH9.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K