2GXQ

HERA N-terminal domain in complex with AMP, crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.8015
Spacegroup name	C 1 2 1
Unit cell lengths	77.582, 52.887, 44.643
Unit cell angles	90.00, 95.26, 90.00

Refinement procedure

Resolution	21.820 - 1.200
R-factor	0.13726
Rwork	0.136
R-free	0.16824
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB-ID 1hv8
RMSD bond length	0.020
RMSD bond angle	1.911
Data scaling software	HKL-2000
Phasing software	COMO
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	53955
<I/σ(I)>	21.2	1.5
Completeness [%]	96.0	50
Redundancy	2.6	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	14% PEG6000, 0.3M CaCl2, 0.1M Tris/HCl pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K