2GXB
Crystal Structure of The Za Domain bound to Z-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.603, 92.776, 50.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.250 |
| R-factor | 0.19908 |
| Rwork | 0.194 |
| R-free | 0.24392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1QBJ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.308 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 7511 | |
| Completeness [%] | 90.7 | 92.5 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 292 | 40% PEG600, 100mM Sodium Acetate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG600 | ||
| 2 | 1 | 1 | Sodium Acetate | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG600 | ||
| 5 | 1 | 2 | Sodium Acetate | ||
| 6 | 1 | 2 | H2O |
