2GXA
Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.510, 100.881, 125.023 |
| Unit cell angles | 92.60, 111.46, 106.01 |
Refinement procedure
| Resolution | 44.650 - 3.150 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | composite of PDB ENTRY 1TUE and unpublished structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.100 | 0.718 |
| Number of reflections | 74589 | |
| <I/σ(I)> | 10.5 | 1.2 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 100 mM KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | KCL | ||
| 4 | 1 | 2 | H2O |
