2GX9

X-ray structure of influenza virus NS1 effector domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2005-12-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97909
Spacegroup name	P 43 2 2
Unit cell lengths	59.000, 59.000, 268.022
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.207
Rwork	0.206
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.654
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Number of reflections	28692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	200 mM NaSCN, 100 mM Hepes pH 6.8, 16% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K