2GX8
The Crystal Structure of Bacillus cereus protein related to NIF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.94642 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.036, 95.036, 260.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.960 - 2.200 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.26123 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.700 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.030 | 0.325 |
| Number of reflections | 68204 | |
| <I/σ(I)> | 34.89 | 3.69 |
| Completeness [%] | 97.0 | 86.7 |
| Redundancy | 5.48 | 3.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES, 0.25M NaCl, 25%w/v PEG1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
