2GX2

Crystal structural and functional analysis of GFP-like fluorescent protein Dronpa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	73.091, 106.160, 110.070
Unit cell angles	61.17, 70.85, 86.21

Refinement procedure

Resolution	19.990 - 1.800
R-factor	0.182
Rwork	0.182
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gx0
RMSD bond length	0.006
RMSD bond angle	1.400
Data scaling software	HKL-2000
Phasing software	X-PLOR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	236555
Completeness [%]	94.4	81.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K