2GWX
MOLECULAR RECOGNITION OF FATTY ACIDS BY PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.772, 94.222, 96.701 |
Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
Rwork | 0.246 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 19.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.080 |
Number of reflections | 40000 |
Completeness [%] | 99.0 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | pH 9.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-tris-propandiol | 40 (mM) | |
2 | 1 | reservoir | dithiothreitol | 10 (mM) | |
3 | 1 | reservoir | propandiol | 2.5 (%) | |
4 | 1 | reservoir | EDTA | 1 (mM) | |
5 | 1 | reservoir | heptyl-beta-D-glucopyranoside | 0.5 (%) | |
6 | 1 | reservoir | 0.2 (mM) | ||
7 | 1 | reservoir | PEG8000 | 5 (%) |