2GWN
The structure of putative dihydroorotase from Porphyromonas gingivalis.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97874, 0.97912 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 86.459, 86.873, 114.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.100 - 1.850 |
R-factor | 0.15099 |
Rwork | 0.149 |
R-free | 0.19372 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.379 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.100 | 1.940 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 36917 | |
<I/σ(I)> | 3.4 | |
Completeness [%] | 99.4 | 99 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 2M ammonium sulfate, 0.1M Cacodylate pH6.5, 0.1M NaCl, glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |