2GW8
Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.945 |
Spacegroup name | P 63 |
Unit cell lengths | 61.141, 61.141, 48.074 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.480 - 1.850 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pil |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.065 | 0.418 |
Number of reflections | 8803 | |
<I/σ(I)> | 28.1 | 99.63 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.17M ammonium sulphate / 25.5% PEG4000 / 15% v/v glycerol, pH Unbuffered, VAPOR DIFFUSION, SITTING DROP, temperature 293K |