2GW3
Crystal structure of stony coral fluorescent protein Kaede, green form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-25 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.496, 80.476, 53.528 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.400 |
| Rwork | 0.197 |
| R-free | 0.21400 |
| Structure solution method | MIR |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 100081 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2M Li2SO4, 2mM NiCl2, 0.1M Tris, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
