2GW1
Crystal Structure of the Yeast Tom70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.880, 168.597, 82.987 |
| Unit cell angles | 90.00, 102.59, 90.00 |
Refinement procedure
| Resolution | 46.170 - 3.000 |
| R-factor | 0.2585 |
| Rwork | 0.259 |
| R-free | 0.31600 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 25274 | |
| Completeness [%] | 95.0 | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25% PEG 4000, 0.1M ammonium citrate, 0.2M ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
