2GVY
Monoclinic crystal form of Aspergillus niger alpha-amylase in complex with maltose at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.451, 101.149, 75.185 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.674 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.987 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | 0.578 |
| Total number of observations | 82342 | |
| Number of reflections | 84823 | |
| <I/σ(I)> | 5.8 | 1.3 |
| Completeness [%] | 96.6 | 95 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 30% PEG 6000, 0.1M ammonium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
