2GVW

Structure of diisopropyl fluorophosphatase (DFPase) holoenzyme (RT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2004-05-25
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.440, 83.290, 87.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.860
R-factor	0.178
Rwork	0.178
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.860
Rmerge	0.058
Number of reflections	25610
<I/σ(I)>	17
Completeness [%]	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K