2GVI
Crystal structure of a putative formylmethanofuran dehydrogenase subunit e (ta1109) from thermoplasma acidophilum at 1.87 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000001 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.680, 97.650, 75.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.080 - 1.870 |
R-factor | 0.19109 |
Rwork | 0.190 |
R-free | 0.21700 |
Structure solution method | MAD, MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.644 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.080 | 30.100 | 1.940 |
High resolution limit [Å] | 1.870 | 4.020 | 1.870 |
Rmerge | 0.114 | 0.024 | 0.698 |
Number of reflections | 24246 | ||
<I/σ(I)> | 8.38 | 25.78 | 1.27 |
Completeness [%] | 99.5 | 99.5 | |
Redundancy | 4.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6 | 277 | 10.0% PEG-8000, 0.2M Zn(OAc)2, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
2 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6 | 277 | 0.2M MgNO3, 20.0% PEG-3350, No Buffer pH 5.8, pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |